CS0013

Red Protease Detection Kit

Fluorescent protease assay, high sensitivity

• Synonym(s): Fluorescent Protease Assay Kit, Protease Assay Kit
• UNSPSC Code: 12350000
• NACRES: NA.51

Properties

• Quality Level: 200
• packaging: pkg of 1 kit
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Red Protease Detection Kit uses TAMRA-casein as a convenient fluorescent substrate for detection of proteases in various samples.

Fluorescence excitation wavelength = 535 nm

Fluorescence emission wavelength = 595 nm

Application

Detection and quantitation of protease activity in samples. Useful for impurity testing.

Features and Benefits

• Fluorescence is pH-independent
• No separation or precipation steps are required
• High sensitivity and signal-to-noise
  

Components

CS0013A Trypsin (1 mg/mL): 100 μL

CS0013B Protease AssayBuffer × 2: 30 mL

CS0013C TAMRA-Casein substrate: 280 μL

Suitability

Detection limit approximately 19 ng/ml, when using a 1 hour incubation with Trypsin as a standard.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable