BXPC3 Cell Line human

93120816

• Synonym(s): Biopsy Xenograft of Pancreatic Carcinoma Cell Line-3, BxPC-3 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human pancreas
• description: Human primary pancreatic adenocarcinoma
• growth mode: Adherent
• karyotype: 2n = 59, near triploid
• morphology: Epithelial
• products: Mucin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human primary pancreatic adenocarcinoma

Cell Line Description

Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma.

Application

BXPC3 cell line has been used to determine the biological and therapeutic importance of gastrin gene expression in pancreatic adenocarcinomas.

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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