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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-IgG, bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against bovine IgG including IgG1 and IgG2. It does not react with bovine IgM.
Immunogen
Purified bovine IgG
Application
Determination of bovine immunoglobulin levels
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation..
Other Notes
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Certificates of Analysis (COA)
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