H9C2 (2-1) Cell Line from rat
88092904, rat cardiovascular , Myoblast
Synonym(s):
H9c2 Cells
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About This Item
UNSPSC Code:
41106514
product name
H9C2 (2-1) Cell Line from rat, 88092904, BDIX heart myoblast
biological source
rat cardiovascular
growth mode
Adherent
karyotype
Not specified
morphology
Myoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat BDIX heart myoblast
Cell Line Description
Skeletal muscle properties. Myotubes formed at confluency respond to acetylcholine. Differentiation improved by reducing serum concentration to 1%.
Application
Differentiation studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Important to passage at sub-confluence, reclone periodically to keep differentiated characteristics. Cells will fuse to form myotubes if allowed to reach confluency.
Other Notes
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Certificates of Analysis (COA)
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