G-361

88030401

• UNSPSC Code: 41106514

Properties

• biological source: human skin
• growth mode: Adherent
• karyotype: Triploid, modal n.o 69
• morphology: Epithelial
• products: Melanin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian malignant melanoma

Cell Line Description

Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

Hormone studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells are slow in attaching.

Other Notes

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