C3367
Anti-CIITA antibody, Mouse monoclonal
clone 7-1H, purified from hybridoma cell culture
Synonym(s):
Anti-C2TA, Anti-MHC Class II Transactivator
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7-1H, monoclonal
form
buffered aqueous solution
mol wt
antigen ~130 kDa
species reactivity
mouse, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
western blot: 2-4 μg/mL using total cell extract of Raji cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CIITA(4261)
mouse ... Ciita(12265)
General description
Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ .
Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ.
Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.
Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.
Application
The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry.
Biochem/physiol Actions
Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Giovanna Barbieri et al.
International immunology, 14(8), 839-848 (2002-07-31)
The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle
H Zhou et al.
Journal of immunology (Baltimore, Md. : 1950), 158(10), 4741-4749 (1997-05-15)
The MHC class II transactivator gene (CIITA) coordinately controls the expression of the three major human class II genes, HLA-DR, HLA-DQ, and HLA-DP. Indeed, patients with one form of MHC class II immunodeficiency disease, due to defective CIITA genes, lack
Isoforms of the class II transactivator protein
Barbieri G, et al.
International Immunology, 14(8), 839-848 (2002)
W Wiszniewski et al.
Journal of immunology (Baltimore, Md. : 1950), 167(3), 1787-1794 (2001-07-24)
The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding
Rodrigo Naves et al.
International immunology, 14(5), 481-491 (2002-04-30)
MHC class II expression defects have been evidenced in several human tumor cell lines originating from lung cancers or retinoblastoma. Accordingly, the mouse adenocarcinoma and fibrosarcoma cell lines, RAG and L(tk-), do not express I-A and I-E molecules even when
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