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A4929

Supelco

N,N′-Bis(acryloyl)cystamine

BioReagent, suitable for electrophoresis

Synonym(s):

2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide

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About This Item

Empirical Formula (Hill Notation):
C10H16N2O2S2
CAS Number:
60984-57-8
Molecular Weight:
260.38
EC Number:
262-546-8
MDL number:
MFCD00036225
UNSPSC Code:
12352116
PubChem Substance ID:
24890926
NACRES:
NA.25

product line

BioReagent

Quality Level

200

form

powder

suitability

suitable for electrophoresis

storage temp.

−20°C

SMILES string

C=CC(=O)NCCSSCCNC(=O)C=C

InChI

1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)

InChI key

DJVKJGIZQFBFGS-UHFFFAOYSA-N

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Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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