88185

Tetrazole solution

suitable for DNA synthesis, filtered through a 1 μm filter, ~0.45 M in acetonitrile

• Synonym(s): 1H-Tetrazole
• Empirical Formula (Hill Notation): CH₂N₄
• CAS Number: 288-94-8
• Molecular Weight: 70.05
• Beilstein: 105799
• MDL number: MFCD00005247
• UNSPSC Code: 12352100
• PubChem Substance ID: 57653295
• NACRES: NA.21

Properties

• form: liquid
• Quality Level: 300
• quality: filtered through a 1 μm filter
• concentration: ~0.45 M in acetonitrile
• technique(s): DNA synthesis: suitable
• impurities: ≤0.003% water
• bp: 84 °C (lit.)
• mp: 156-158 °C (lit.)
• density: 0.798 g/mL at 20 °C
• SMILES string: c1nnn[nH]1
• InChI: 1S/CH2N4/c1-2-4-5-3-1/h1H,(H,2,3,4,5)
• InChI key: KJUGUADJHNHALS-UHFFFAOYSA-N

Description

General description

The product is ~0.45M solution of tetrazole in acetonitrile. Tetrazole ring plays significant role in various synthetic and industrial processes.

Application

Tetrazole solution is useful for DNA synthesis. It may be employed as a catalyst for the in situ synthesis of deoxyribonucleoside phosphoramidites. It may be used in the preparation of following anionic nucleotide-lipids:

• thymidine 3′-(1,2-dilauroyl-sn-glycero-3-phosphate) , diC₁₂-3′-dT
• thymidine 3′-(1,2-dimyristoyl-sn-glycero-3-phosphate) diC₁₄- 3′-dT
• thymidine-3′-(1,2-dipalmitoyl-sn-glycero-3-phosphate) diC₁₆- 3′-dT

Caution

Saturated solution at room temperature; storage below room temperature causes precipitation.

Other Notes

Catalyst used in the phosphite triester method of oligonucleotide synthesis for the coupling of (dialkylamino)phosphines

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H302 + H312 + H332,H319
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 41.0 °F - closed cup
• Flash Point(C): 5 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves