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Ham′s Nutrient Mixture F12

liquid

Synonym(s):

Nutrient Mixture F12 Ham’s

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use
with 1.0 mM L-glutamine

sterility

sterile

form

liquid

quality

for further manufacturing use

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: 1176 mg/L
sodium pyruvate: 110.1 mg/L
phenol red: 1.274 mg/L
L-glutamine: 146.2 mg/L

shipped in

ambient

storage temp.

2-8°C

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Application

Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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S Mahmoudi et al.
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Olga Blaževitš et al.
International journal of molecular sciences, 21(7) (2020-04-01)
The circadian transcriptional network is based on a competition between transcriptional activator and repressor complexes regulating the rhythmic expression of clock-controlled genes. We show here that the MYC-associated factor X, MAX, plays a repressive role in this network and operates

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