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Sigma-Aldrich

4-Methylumbelliferyl butyrate

lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence

Synonym(s):

MU-Bu

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About This Item

Empirical Formula (Hill Notation):
C14H14O4
CAS Number:
17695-46-4
Molecular Weight:
246.26
Beilstein:
227973
EC Number:
241-694-7
MDL number:
MFCD00038087
UNSPSC Code:
12352204
PubChem Substance ID:
57647789
NACRES:
NA.32

product name

4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)

Assay

≥95% (HPCE)

form

powder or crystals

mp

90-92 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 312 nm in methanol
λex 360 nm; λem 449 nm (Reaction product)
λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

InChI key

WKPUJZVCZXWKCK-UHFFFAOYSA-N

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Application

suitable as fluorogenic substrate for esterases/lipases

Other Notes

Fluorogenic substrate for butyrate esterase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of lipase activity using image analysis.
Marcelo Müller-Santos et al.
Analytical biochemistry, 351(2), 305-307 (2006-03-10)
Ivan Kurtovic et al.
Fish physiology and biochemistry, 36(4), 1041-1060 (2010-02-10)
Lipases were purified from delipidated pyloric ceca powder of two New Zealand-sourced fish, Chinook salmon (Oncorhynchus tshawytscha) and hoki (Macruronus novaezelandiae), by fractional precipitation with polyethylene glycol 1000, followed by affinity chromatography using cholate-Affi-Gel 102, and gel filtration on Sephacryl
M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Daniel Kübler et al.
European biophysics journal : EBJ, 44(8), 655-665 (2015-08-01)
Because of their vast diversity of substrate specificity and reaction conditions, lipases are versatile materials for biocatalysis. Lipase A from Bacillus subtilis (BSLA) is the smallest lipase yet discovered. It has the typical α/β hydrolase fold but lacks a lid
E Speeleveld et al.
Journal of clinical microbiology, 32(5), 1362-1363 (1994-05-01)
Moraxella catarrhalis can easily be differentiated from other oxidase-positive, gram-negative cocci with tributyrine, 4-methylumbelliferyl butyrate, or indoxyl acetate. All M. catarrhalis give positive reactions, and all Neisseria spp. give negative reactions. The 4-methylumbelliferyl butyrate tube test and indoxyl acetate strip
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