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67996

Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

for molecular biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Components

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCG8073
BCCF1926
BCCC4245
BCCB8721
BCBX7593

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Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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