16669

Magenta-Gal

reagent for selection of recombinant bacterial clones

• Synonym(s): 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal
• Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
• CAS Number: 93863-88-8
• Molecular Weight: 408.63
• Beilstein: 1552602
• MDL number: MFCD00210022
• UNSPSC Code: 12352200
• PubChem Substance ID: 329751317

Properties

• grade: for molecular biology
• Quality Level: 100
• sterility: non-sterile
• Assay: ≥98.0% (HPLC)
• form: powder
• solubility: DMF: soluble; DMSO: soluble
• suitability: suitable for β-galactosidase test
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11-,12+,13-,14-/m1/s1
• InChI key: CHRVKCMQIZYLNM-RKQHYHRCSA-N

Description

General description

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

Principle

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.