G6PDHII-RO

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade II, from yeast

• Synonym(s): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: yeast
• Quality Level: 100
• form: suspension
• specific activity: ~140 units/mg protein (At 25 °C with glucose-6-P as the substrate; standardized with BSA.)
• packaging: pkg of 2 mL (10127671001 [5 mg/mL]); pkg of 5 mL (10737232001 [5 mg/mL])
• manufacturer/tradename: Roche
• optimum pH: 9.2
• pH range: 5-11
• shipped in: wet ice

Description

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.

Application

Glucose-6-Phosphate Dehydrogenase (G6P-DH) is a component of cofactor recycling systems for NADPH. Additional reagent glucose-6-phosphate.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Quality

Contaminants: <0.001% CK, <0.05% GR, <0.01% 6-PGDH and PGluM each, <0.02% HK, <0.002% PGI

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.0

Preparation Note

Activator: Mg²⁺ (5 to 10 mM)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash