10269611001
Roche
Neuraminidase (Sialidase)
from Arthrobacter ureafaciens
Synonym(s):
PCR, taq
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About This Item
Recommended Products
biological source
bacterial (Arthrobacter ureafaciens)
Quality Level
form
solution
specific activity
~25 units/mg protein
packaging
pkg of 1 U (100 μl)
manufacturer/tradename
Roche
optimum pH
5.0-5.5
storage temp.
2-8°C
General description
Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Specificity
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.
Application
- cell surface lectin array analysis.
- hemagglutination assays.
- cell adhesion assay.
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:
- detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells
- release of sialic acid from cells
- antibody-overlay lectin microarray
Physical properties
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
Physical form
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
Preparation Note
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
A high-throughput capillary isoelectric focusing immunoassay for fingerprinting protein sialylation.
Lam Raga Anggara Markely et al.
Biotechnology progress, 32(1), 235-241 (2015-11-21)
The serum half-life, biological activity, and solubility of many recombinant glycoproteins depend on their sialylation. Monitoring glycoprotein sialylation during cell culture manufacturing is, therefore, critical to ensure product efficacy and safety. Here a high-throughput method for semi-quantitative fingerprinting of glycoprotein
Sialylation by ??galactoside ??2,6?sialyltransferase and N?glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma
Suzuki O, et al.
International Journal of Oncology, 46(3), 973-980 (2015)
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Dendritic cell immunoreceptor (DCIR) is a C-type lectin receptor with a carbohydrate recognition domain and an immunoreceptor tyrosine-based inhibitory motif. Previously, we showed that Dcir-/- mice spontaneously develop autoimmune enthesitis and sialadenitis, and also develop metabolic bone abnormalities. However, the
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The human JC polyomavirus (JCV) is the etiologic agent of the fatal central nervous system (CNS) demyelinating disease progressive multifocal leukoencephalopathy (PML). PML typically occurs in immunosuppressed patients and is the direct result of JCV infection of oligodendrocytes. The initial
Protocols
Using Neuraminidase to remove sialic acid from purified lipooligosaccharide (LOS)
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