10108626001

Poly(A)

lyophilized, suitable for PCR, pkg of 100 mg

• Synonym(s): Poly(A), Polyadenylic acid
• UNSPSC Code: 41105600

Properties

• description: Polyadenylic acid
• Quality Level: 100
• form: lyophilized
• mol wt: 700-3500 kDa
• packaging: pkg of 100 mg
• manufacturer/tradename: Roche
• concentration: 0.5 mg/mL (Working concentration)
• technique(s): PCR: suitable
• color: white
• solubility: water: soluble
• absorbance ratio: A_{290/260 nm} 0.03-0.05; A_{280/260 nm} 0.28-0.32; A_{250/260 nm} 0.86-0.90
• storage temp.: 2-8°C

Description

General description

Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.

Application

Poly(A) has been used for droplet digital PCR (ddPCR) assay.

Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.

Biochem/physiol Actions

Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.

Quality

Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀

Sequence

Chain Length 2.100 to 10.000 nucleotides

Unit Definition

1 A₂₆₀ unit corresponds to 40 μg ssRNA.

Physical form

Lyophilizate, potassium salt

Preparation Note

Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable