MABT913

Anti-Cytokeratin 19 Antibody, clone TROMA-3

clone TROMA-3, from rat

• Synonym(s): Keratin, type I cytoskeletal 19, CK-19, Keratin-19, K19
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: TROMA-3, monoclonal
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): electron microscopy: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_032497
• UniProt accession no.: P19001
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... KRT19(3880) ; mouse ... Krt19(16669)

Description

General description

Keratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.

Specificity

Clone TROMA-3 detects cytokeratin 19 in human and murine cells.

Immunogen

Purified intermediate filaments from DM1 (Trophoblatoma) cell line.

Application

Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.

Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).

Quality

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.

Target description

~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1