MABT129
Anti-VE-Cadherin Antibody (CD144), clone BV9
clone BV9, from mouse
Synonym(s):
Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
BV9, monoclonal
species reactivity
human
technique(s)
activity assay: suitable
immunocytochemistry: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDH5(1003)
General description
Also known as CD144, human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell to cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherins are reported to play an important role in angiogeneis and in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.
Specificity
This antibody recognizes the extracellular domain of VE-Cadherin.
Immunogen
Epitope: Extracellular domain.
Recombinant protein corresponding to the extracellular domain of human VE-Cadherin.
Application
Activity Assay Analysis: A representative lot from an independent laboratory increased endothelial permeability, inhibited the organization of vasclular-like structures, and prevented the protective effect of VEGF against apoptosis in endothelial cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This VE-Cadherin antibody is validated for use in ICC & Enzyme Assay for the detection of the VE-Cadherin protein.
Quality
Evalutated by Immunocytochemistry in HUVEC cells.
Immunocytochemistry Analysis: 1 µg/mL of this antibody detected VE-Cadherin in HUVEC cells.
Immunocytochemistry Analysis: 1 µg/mL of this antibody detected VE-Cadherin in HUVEC cells.
Target description
88 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing PBS without 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HUVEC cells
HUVEC cells
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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A novel endothelial-specific membrane protein is a marker of cell-cell contacts.
Lampugnani, MG; Resnati, M; Raiteri, M; Pigott, R; Pisacane, A; Houen, G; Ruco, LP; Dejana, E
The Journal of cell biology null
L Caveda et al.
The Journal of clinical investigation, 98(4), 886-893 (1996-08-15)
Endothelial cell proliferation is inhibited by the establishment of cell to cell contacts. Adhesive molecules at junctions could therefore play a role in transferring negative growth signals. The transmembrane protein VE-cadherin (vascular endothelial cadherin/cadherin-S) is selectively expressed at intercellular clefts
Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin.
Breviario, F; Caveda, L; Corada, M; Martin-Padura, I; Navarro, P; Golay, J; Introna et al.
Arteriosclerosis, Thrombosis, and Vascular Biology null
Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability.
Corada, M; Liao, F; Lindgren, M; Lampugnani, MG; Breviario, F; Frank, R; Muller et al.
Blood null
Cécile Otten et al.
EMBO molecular medicine, 10(10) (2018-09-06)
Cerebral cavernous malformations (CCMs) are vascular lesions in the central nervous system causing strokes and seizures which currently can only be treated through neurosurgery. The disease arises through changes in the regulatory networks of endothelial cells that must be comprehensively
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