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MABD90

Sigma-Aldrich

Anti-HLA-DRB1 Antibody, clone DJ9

clone DJ9, from mouse

Synonym(s):

Major histocompatibility complex, class II, DRB1, DRB1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DJ9, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_002112

UniProt accession no.

P01911

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HLA-DRB1(3123)

General description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells and presents them on the cell surface for recognition by the CD4 T-cells. Class II molecules are expressed in antigen presenting cells B lymphocytes, dendritic cells, macrophages. HLA-Drb1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. HLA-DRB1, clone DJ9, has been shown to be a relevant marker of bone marrow derived mesenchymal stem cells and their osteogenic derivatives (Andersen, D., et al., 2011).

The previously assigned protein identifier Q5Y7A7 has been merged into P01911. Full details can be found on the UniProt database.

Specificity

This antibody recognizes human HLA-DRB1.

Immunogen

Whole hMSC-TERT Cells

Application

Detect HLA-DRB1 using this Anti-HLA-DRB1 Antibody, clone DJ9 validated for use in Western Blotting, ICC, IHC, Flow Cytometry.
Research Category
Inflammation & Immunology

Stem Cell Research
Research Sub Category
Immunological Signaling

Mesenchymal Stem Cells
This antibody has been shown to work in Immunocytochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Immunohistochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Flow Cytometry (Andersen, D., et al. (2011)).

Quality

Evaluated by Western Blot in Daudi cell lysates. Western Blot Analysis: A 1:100,000 dilution of this antibody detected HLA-DRB1 in Duadi cell lysates.

Target description

~30 kDa observed

Physical form

Format: Purified

Analysis Note

Control
Daudi cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Takamichi Miyazaki et al.
Genesis (New York, N.Y. : 2000), 52(1), 49-55 (2013-11-21)
Human pluripotent stem cells (hPSCs) have the potential for unlimited expansion and differentiation into cell types of all three germ layers. Cryopreservation is a key process for successful application of hPSCs. However, the current conventional method leads to poor recovery

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