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MAB1273C3

Sigma-Aldrich

Anti-Mitochondria Antibody, clone 113-1, Cy3 Conjugate

clone 113-1, from mouse, CY3 conjugate

Synonym(s):

Human Mitochondria

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

113-1, monoclonal

species reactivity

human (mitochondria protein)

should not react with

rat (mitochondria protein), mouse (mitochondria protein)

technique(s)

immunocytochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

General description

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273C3 recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Specificity

This antibody recognizes human. Antibody is not reactive with mouse or rodent.

Immunogen

Surface of intact human mitochondria

Application

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1 & Cy3 Conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).
Research Category
Stem Cell Research

Cell Structure
Research Sub Category
Developmental Neuroscience

Organelle & Cell Markers

Quality

Evaluated by Immunocytochemistry in neonatal human fibroblasts cells (SCC058).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in neonatal human fibroblast cells (SCC058).

Target description

60 kDa calculated

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Storage and Stability

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Neonatal human fibroblasts cells (SCC058).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Roza K Przanowska et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7687-7702 (2020-04-12)
miR-206, miR-1a-1, and miR-1a-2 are induced during differentiation of skeletal myoblasts and promote myogenesis in vitro. miR-206 is required for skeletal muscle regeneration in vivo. Although this miRNA family is hypothesized to play an essential role in differentiation, a triple

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