MAB1012-I

Anti-Alkaline Phosphatase Antibody, E. coli

• Synonym(s): EC:3.1.3.1, JW0374, alkaline phosphomonoesterase, b0383, glycerophosphatase, phosphomonoesterase
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: N/A, monoclonal
• mol wt: calculated mol wt 49.44 kDa; observed mol wt ~50 kDa
• purified by: using protein G
• species reactivity: E. coli
• packaging: antibody small pack of 100
• technique(s): western blot: suitable
• isotype: IgG2bκ
• epitope sequence: Unknown
• Protein ID accession no.: NP_414917
• UniProt accession no.: P00634
• storage temp.: 2-8°C

Description

Specificity

This mouse recombinant monoclonal antibody detects Alkaline Phosphatase from E. coli.

Immunogen

Purified Alkaline Phosphatase from E. coli.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from E. coli.

Western Blotting Analysis: A 1:1,000 Dilution of this antibody detected Alkaline Phosphatase in E. coli lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Alkaline Phosphatase in Western Blotting application (Yogev, D., et al. (1991). J Bacteriol. 173(6):2035-44; Taw, M.N., et al. (2022). Sci Rep. 12(1):14862).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Alkaline phosphatase (UniProt: P00634; also known as EC:3.1.3.1, APase) is encoded by the phoA (also known as b0383, JW0374) gene (Gene ID: 945041) in E. coli. Alkaline phosphatase is a dimeric, non-glycosylated metalloenzyme, requiring two zinc ions (Zn²⁺) and one magnesium ion (Mg²⁺) per subunit for its activity and structural stability. The active site of Alkaline phosphatase includes a serine residue that plays a crucial role in its catalytic mechanism. The primary function of E. coli Alkaline phosphatase is to catalyze the hydrolysis of phosphate monoesters, resulting in the release of an inorganic phosphate and an alcohol. The enzyme is involved in various processes, including phosphate metabolism and regulation. Alkaline phosphatase is also known for its ability to transfer phosphate groups to acceptor molecules, which is key in its role in transphosphorylation reactions. Alkaline Phosphatase has a wide range of applications in molecular biology. It is used as a reporter enzyme in various assays, including ELISA and Western blotting. During molecular cloning, it is commonly used to dephosphorylate the 5′ ends of DNA fragments to prevent self-ligation during cloning procedures.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable