LIF2050
Leukemia Inhibitory Factor from mouse
50 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
Synonym(s):
LIF, Differentiation-stimulating Factor, D Factor
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.81
Recommended Products
biological source
mouse
Quality Level
Assay
>95% (SDS-PAGE)
form
liquid
specific activity
≥1 x 10(E8) U/mg
manufacturer/tradename
Chemicon®
concentration
100 μg/mL
technique(s)
cell culture | stem cell: suitable
impurities
<0.1 ng/mg Endotoxin (of LIF)
input
sample type: mouse embryonic stem cell(s)
sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
General description
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 22 kDa protein containing 203 amino acid residues.
Product Source: mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by multiple FPLC steps.
Application
50 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
QC PROTOCOL
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 300 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 300 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Research Category
Stem Cell Research
Stem Cell Research
Unit Definition
Specific Activity: where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
Physical form
Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservatives added.
Storage and Stability
Maintain at 2-8°C for 6-12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
Analysis Note
Specific Activity: The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells.The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL
Tested negative in both aseptic and mycoplasmic tests.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Articles
Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).
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