AP506P
Goat Anti-Human Ig λ chain Antibody, HRP conjugate
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity purified immunoglobulin
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Specificity
Reacts with human lambda light chains. Absorbed for human myeloma proteins with kappa light chains.
The antibody reacts with human lambda light chains as demonstrated by ELISA and flow cytometry.
Immunogen
Epitope: Ig lambda light chain
Prepared from pooled antisera from goats hyperimmunized with human myeloma proteins with lambda light chains.
Application
ELISA: 1:4,000-1:8,000.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Goat anti-Human Ig λ chain Antibody, HRP conjugate detects level of Human Ig λ chain & has been published & validated for use in ELISA.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Physical form
Affinity Chromatography
Purified by affinity chromatography on pooled human Igs with lambda light chains covalently linked to agarose. Liquid in 50% glycerol/50% PBS, pH 7.4.
Storage and Stability
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Siqi Gong et al.
Antibodies (Basel, Switzerland), 11(2) (2022-06-02)
When constructing isogenic recombinant IgM-IgG pairs, we discovered that μ heavy chains strongly prefer partnering with λ light chains for optimal IgM expression in transiently cotransfected Expi293 cells. When μ chains were paired with κ light chains, IgM yields were
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