69449
BL21 Competent Cells - Novagen
BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.
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About This Item
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biological source
Escherichia coli
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: >2×107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Related Categories
General description
BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Components
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlBL21 Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
•2 × 0.2 ml5 × 0.2 mlBL21 Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Other Notes
1. Phillips, T. A., Van Bogelen, R. A., and Neidhardt, F. C. (1984) J. Bacteriol.
159, 283–287.
159, 283–287.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Developmental cell, 41(2), 143-156 (2017-04-26)
The spindle assembly checkpoint kinase Mps1 not only inhibits anaphase but also corrects erroneous attachments that could lead to missegregation and aneuploidy. However, Mps1's error correction-relevant substrates are unknown. Using a chemically tuned kinetochore-targeting assay, we show that Mps1 destabilizes microtubule attachments
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