13-173

MBP, recombinant

• Synonym(s): myelin basic protein
• UNSPSC Code: 12352200
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: bovine
• Quality Level: 100
• form: liquid
• mol wt: M_w ~47 kDa
• manufacturer/tradename: Upstate^®
• concentration: 1 mg/mL
• technique(s): activity assay: suitable
• NCBI accession no.: NM_001025081.1; NM_001025090.1; NM_001025092.1; NM_001025094.1; NM_001025098.1; NM_001025100.1; NM_001025101.1; NM_002385.2
• UniProt accession no.: P02686
• shipped in: dry ice

Description

General description

Product Source: Recombinant fusion protein containing bovine myelin basic protein (MBP) with an N-terminal GST tag and a C-terminal 6His tag, expressed in E. coli.

Quality

Routinely evaluated by kinase assay

Physical form

500 µg recombinant MBP in 500 µl of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 500 mM imidazole, 1 mM PMSF, and 10% glycerol. Frozen solution.

ProBond Resin chromatography

Storage and Stability

Stable for 2 years at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Specific Activity: This lot was successfully phosphorylated in a non-radioactive kinase assay using recombinant active MAP Kinase 2/Erk2 (Catalog # 14-173). MBP phosphorylation was detected by immunoblot analysis using anti-phospho-MBP (Catalog # 05-429). Immunoblot shown to the right.

Other Notes

Purity information is lot specific, please consult the certificate of analysis

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable