1.12535

GranuCult^® prime Peptone salt solution (Maximum recovery diluent)

according to ISO 6887, according to ISO 8199, for heterotrophic bacteria

• Synonym(s): MRD, Maximum recovery diluent, Peptone solution, Saline Peptone Solution, Saline Peptone Water, Sodium Chloride Peptone Solution, Sodium Chloride Peptone Water
• UNSPSC Code: 41106213

Properties

• Agency: according to ISO 6887; according to ISO 8199
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® prime
• storage condition: protect from light (Keep tightly closed and dry.)
• technique(s): microbiological culture: suitable
• color: light brown
• pH: 6.8-7.2 (25 °C, 9.5 g/L in H₂O, after autoclaving)
• solubility: 9.5 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: heterotrophic bacteria

Description

General description

GranuCult^® prime Peptone salt solution, also known as Maximum Recovery diluent, is of isotonic strength to ensure the recovery of organisms from various sources. It combines the protective effect of peptone in the diluent with the osmotic support of physiological saline. Within 1-2 hours of dilution of the sample, there is no multiplication of organisms due to the low concentration of peptones. Peptone salt solution complies with the recommendations of ISO 6887 and the German 35 Lebensmittelgesetz (German food law). This diluent can be used as an alternative to RINGER solution for milk and liquid milk products, dried milk, cheese, butter, meat and meat products, ice cream, and chilled food based on milk.

Application

GranuCult^® prime Peptone salt solution is suitable for the preparation of an isotonic diluent for maximum recovery of organisms, especially in milk and meat testing.

Features and Benefits

• GranuCult^® offers superior granulated culture media.
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions.
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (color): colourless to slightly colourless
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): 70 - 130 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): 70 - 130 %
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): 70 - 130 %
Incubation: 45 minutes up to 1 hour at 20 - 25 °C
Reference medium: Tryptic Soy Agar 18 -24 hours at 35 - 37 °C, aerobic

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1