1.02129

Chromolith^® RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

• Synonym(s): Chromolith^® Performance RP-18 endcapped 100-4.6 HPLC column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Chromolith^® RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column
• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 200 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 4.6 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® Performance RP-18 endcapped HPLC columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) and the surface modification is octadecylsilane with full end-capping. Chromolith^® Performance RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column.

Chromolith^® Performance RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:

• Rapid separations at very low column back-pressure
• High matrix tolerance, allowing time and cost savings with simplified workflows
• Cost savings due to extended column lifetimes
• Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith^® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032] Or Chromolith^® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451] with the Chromolith^® Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]

The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith^® HighResolution RP-18 endcapped 100-4.6 HPLC columns [1.52022] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application

• Salt pollution reduces turbidity, dissolved organic matter, and cyanobacteria in experimental vernal pool communities: Discusses environmental applications of calcium chloride, focusing on its use in managing ecosystems affected by salt pollution, demonstrating its role in environmental remediation and management. (Vigil and Schuler, 2024).

Analysis Note

Appearance: conforms
Carbon content: 17,0 - 18,6 %
Pressure: ≤ 30 bar
stability testing: conforms
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3,9 - 4,6
Tailing (USP): 0,90 - 1,60 %
Selectivity
separation factor (anthracene/progesterone): 1,95 - 2,35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13