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Key Documents

04-080

Sigma-Aldrich

Anti-BAF250a/ARID1a Antibody, clone PSG3

clone PSG3, Upstate®, from mouse

Synonym(s):

Anti-B120, Anti-BAF250, Anti-BAF250a, Anti-BM029, Anti-C1orf4, Anti-CSS2, Anti-ELD, Anti-MRD14, Anti-OSA1, Anti-P270, Anti-SMARCF1, Anti-hELD, Anti-hOSA1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PSG3, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_006015.4
NM_139135.2

UniProt accession no.

O14497

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ARID1A (8289)

Specificity

BAF250a/ARID1a

Immunogen

GST fusion protein corresponding to 419 residues in the mid-portion of human BAF250a/ARID1a

Application

Anti-BAF250a/ARID1a Antibody, clone PSG3 is an antibody against BAF250a/ARID1a for use in WB, ChIP, IC & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Transcription Factors

Quality

Routinely evaluated by immunoblot.

Target description

~270 kDa

Physical form

100 μg of Protein A purified mouse monoclonal IgG in 100μl of 1X PBS, pH 7.0, 0.1% Azide.
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Essential role of ARID2 protein-containing SWI/SNF complex in tissue-specific gene expression.
Xu, F; Flowers, S; Moran, E
The Journal of Biological Chemistry null
Zuqin Nie et al.
Molecular and cellular biology, 23(8), 2942-2952 (2003-04-01)
The SWI/SNF family of chromatin-remodeling complexes has been discovered in many species and has been shown to regulate gene expression by assisting transcriptional machinery to gain access to their sites in chromatin. Several complexes of this family have been reported
Xiaomei Wang et al.
The Biochemical journal, 383(Pt 2), 319-325 (2004-06-02)
p270 (ARID1A) is a member of the ARID family of DNA-binding proteins and a subunit of human SWI/SNF-related complexes, which use the energy generated by an integral ATPase subunit to remodel chromatin. ARID1B is an independent gene product with an
Hiroko Inoue et al.
The Journal of biological chemistry, 277(44), 41674-41685 (2002-08-30)
The mammalian SWI/SNF-related complexes facilitate gene transcription by remodeling chromatin using the energy of ATP hydrolysis. The recruitment of these complexes to promoters remains poorly understood and may involve histone modifications or direct interactions with site-specific transcription factors or other
Guillermo Pablo Vicent et al.
The Journal of biological chemistry, 285(4), 2622-2631 (2009-11-27)
Steroid hormones induce transcription of their responsive genes by complex mechanisms including synergism between the hormone receptors and other transcription factors. On the mouse mammary tumor virus (MMTV) promoter progesterone induction is mediated by the reciprocal synergism between progesterone receptor
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