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739936

Sigma-Aldrich

3-epi-25-Hydroxyvitamin D3 solution

100 μg/mL in ethanol, 98% (CP)

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About This Item

Empirical Formula (Hill Notation):
C27H44O2
CAS Number:
73809-05-9
Molecular Weight:
400.64
EC Number:
200-578-6
MDL number:
MFCD11656130
UNSPSC Code:
12352200
PubChem Substance ID:
329765390
NACRES:
NA.24

Quality Level

200

Assay

98% (CP)

form

solution

concentration

100 μg/mL in ethanol

technique(s)

mass spectrometry (MS): suitable

color

colorless

mp

-144.0 °C

application(s)

detection

storage temp.

−20°C

SMILES string

C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2\C(CCC[C@]12C)=C\C=C3\C[C@H](O)CCC3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23-,24-,25+,27-/m1/s1

InChI key

JWUBBDSIWDLEOM-UEGFJKGBSA-N

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Application

3-epi-25-Hydroxyvitamin D3 abbreviated as 3-epi-25(OH)D3 is a metabolite of vitamin D3 produced in the liver. 3-epi-25(OH)D3 is used as a biomarker to determine the status of vitamin D in the body. It can be also employed as an analytical standard for the quantification of 3-epi-25(OH)D3 molecules in human serum using the LC-MS/MS method.

Packaging

1 mL in ampule

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Description
Pricing

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Full-scan mass spectral evidence for 3-epi-25-hydroxyvitamin D3 in serum of infants and adults
Stepman HCM, et al.
Clinical Chemistry and Laboratory Medicine, 49(2), 253-256 (2011)
Juan Le et al.
Journal of lipid research, 60(5), 1058-1064 (2019-03-25)
Vitamin D metabolite analysis possessed significant clinical value for the pediatric department. However, invasive venipuncture sampling and high blood consumption inflicted much suffering on patients. For alleviation, we carried out a LC-MS method for 25-hydroxyvitamin D quantification in only 3
Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum
van den Ouweland JMW, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 967, 195-202 (2014)

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LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5

This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.

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LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 (Monitored Ions)

The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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