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G005749

HPLC Analysis of Taxols on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 0.3 mL/min
column temp.: 30 °C
detector: UV, 227 nm
injection: 2 μL
sample: 25 mg/L in 70:30, water:methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of taxols.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Baccatin III, ≥95% (HPLC)
    10-Deacetylpaditaxel
    Cephalomannine, ≥97% (HPLC), solid
    10-Deacetyl-7-epipaclitaxel
    Paclitaxel, from Taxus yannanensis, powder
    7-Epipaclitaxel

analytical column

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Description
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standard

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