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Key Documents

G001833

HPLC Analysis of Elastase on Discovery® BIO PolyMA-SCX, Ion Exchange

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5cm × 4.6mm, 5μm particles (59601-U)
mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0
gradient: 0 to 100% B in 30 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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