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21278-U

Supelco

GC Stationary Phase

phase OV-275, bottle of 5 g

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About This Item

UNSPSC Code:
23151817
NACRES:
SB.54

packaging

bottle of 5 g

parameter

25-250 °C temp. range

technique(s)

gas chromatography (GC): suitable

matrix active group

OV-275 phase

solubility

acetone: soluble

column type

packed GC

General description

GC methods are divided into two classes depending on the nature of stationary phases; gas-solid chromatography (GSC) and gas-liquid chromatography (GLC). GSC has solid adsorptive material and solute particles are removed from mobile phase by electrostatic forces. GLC has a thin layer of liquid coated or bonded on the surface of an inert particle or on the walls of the column where solute particles are retained in the liquid phase based on their partition coefficients. The primary necessity of a stationary phase is to provide sample separation sustaining phase integrity over a reasonable period of time. It should be stable for the chemical and thermal changes. Selectivity, peak symmetry, analysis time, degree of separation, peak tailing are a few parameters that should be considered in order to choose a stationary phase. OV-275 is a highly polar silicone.
Synthesized specifically to be purer, of narrow molecular weight range, and without trace catalysts or impurities for use as a GC stationary phase.

Application

OV-275 on Chromosorb WAW packed glass column is suitable for analysis of fatty acid composition of total lipid and major lipid classes, using gas-liquid chromatography.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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M I Aveldaño et al.
Clinical chemistry, 42(3), 454-461 (1996-03-01)
Blood cell and plasma lipid classes and their fatty acids were analyzed in a child with X-linked adrenoleukodystrophy. The increase in saturated fatty acids with very long chains typical of this disease occurred almost exclusively in sphingomyelin. In this lipid
Satinder Ahuja
Chromatography and Separation Science, 139-139 (2003)
James P. Lodge, Jr.
Methods of Air Sampling and Analysis, 98-98 (1988)
David B. Troy, Paul Beringer
Remington: The Science and Practice of Pharmacy, 605-605 (2006)

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