ZMS1150

Anti-MBNL1 Antibody, clone 4A8 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Muscleblind-like protein 1, Triplet-expansion RNA-binding protein
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4A8, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 41.82 kDa; observed mol wt ~40 kDa
• purified by: using protein G
• species reactivity: human, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: NP_997176
• UniProt accession no.: Q9NR56
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... MBNL1(4154)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 4A8 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Muscleblind-like protein 1 (MBNL1).

Immunogen

Full length recombinant protein corresponding to human MBNL1.

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MBNL1 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBNL1 in C6 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MBNL1 in HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MBNL1 in Human esophagus tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Muscleblind-like protein 1 (UniProt: Q9NR56; also known as Triplet-expansion RNA-binding protein) is encoded by the MBNL1 (also known as EXP, KIAA0428, MBNL) gene (Gene ID: 4154) in human. MBNL1 is an RNA splicing protein that mediates pre-mRNA alternative splicing regulation. It can act either as activator or repressor of splicing on specific pre-mRNA targets. It is highly expressed in cardiac and skeletal muscle and during myoblast differentiation and is weakly expressed in other tissues. It is shown to inhibit cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. It regulates the TNNT2 exon 5 skipping through competition with U2 auxiliary factor 2 (U2AF2). MBNL1 binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly and inhibits the formation of spliceosome A.  MBNL1 gene is one of the most consistently overexpressed genes in MLL-rearranged leukemia and its loss significantly impairs propagation of murine and human MLL-rearranged leukemia in vitro and in vivo. Mutations in MBNL1 gene have been linked to dystrophia myotonica that is characterized by myotonia and muscle wasting in the distal extremities. Seven different isoforms of MBNL1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Itskovich, SS., et al. (2020). Nat. Commun. 11; Article 2369; Sznajder, LJ., et al. (2020). Nat. Commun. 11; Article 2022).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable