U6385

Ubiquitin-Specific Protease 14 human

recombinant, expressed in Sf9 cells, ≥75% (SDS-PAGE)

• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Sf9 cells
• Quality Level: 200
• Assay: ≥75% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: 57 kDa
• NCBI accession no.: NM_005151
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), amino acids 2-494 (end) with an N-terminal FLAG-tag, expressed in a baculovirus-infected Sf9 cell expression system.
Ubiquitin-Specific Protease 14 is a deubiquitinating protein responsible for the removal of ubquitin tags attached to proteins by α•NH-peptide bonds and the ε-NH-isopeptide bond.

USP14 has been shown to be phosphorylated by AKT which is required for protein stability and nuclear localization.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Solution in 40 mM Tris-HCl, 110 mM NaCl, 2.2 mM KCl, 80 ng/μl Flag peptide, 3 mM DTT, and 20% Glycerol at pH 8.0.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable