SRP6546

Procathepsin L human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): CTSL, CTSL1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• mol wt: 39.4 kDa (18-333 aa + N-terminal poly-his tag.)
• packaging: pkg of 1 mg; pkg of 100 μg
• concentration: 1 mg/mL
• UniProt accession no.: P07711
• shipped in: wet ice
• storage temp.: −70°C
• Gene Information: human ... CTSL1(1514)

Description

General description

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

Physical form

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable