SRP6519

Lactoferrin from human milk

≥95% (SDS-PAGE)

• Synonym(s): Lactotransferrin, Talalactoferrin
• UNSPSC Code: 12352202

Properties

• biological source: human milk
• Assay: ≥95% (SDS-PAGE)
• form: frozen liquid
• mol wt: 82.4 kDa
• packaging: pkg of 10 mg
• technique(s): ELISA: suitable
• UniProt accession no.: 4057
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... LTF(4057)

Description

General description

Lactoferrin gene is mapped to human chromosome 3p21.31.

Lactoferrin is a glycoprotein present in exocrine secretions and in the secondary granules of polymorphonuclear neutrophils. It is the iron binding protein in milk. Human lactoferrin belongs to the family of transferrins. It is found to be expressed in several glandular epithelial cells and skin epidermal keratinocytes.

Application

Lactoferrin from human milk has been used as a standard in standard curve generation for quantifying recombinant lactoferrin in enzyme-linked immunosorbent assay (ELISA). It has also been used as a positive control in sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE ).

Biochem/physiol Actions

Lactoferrin is known to regulate intestinal iron absorption, and to participate in the defense against bacteria. It functions in the innate immune system via the activation of nuclear factor-κB (NF-κB). This activation occurs via Toll-like receptor 4 pathway due to the interference of lactoferrin with the lipopolysaccharide-stimulated TLR4 signaling. It serves as a ligand for low-density lipoprotein receptor-related protein 1 (LRP1) in human skin keratinocytes and fibroblasts. It is capable of stimulating cell growth in various cell lines. Lactoferrin is also found to participate in wound healing.

Physical form

Frozen in 50 mM Tris and 0.2 M NaCl, pH 8.0 .

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable