SRP5118

PDE4B (305-end), active, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): DKFZp686F2182, DPDE4, MGC126529, PDE4B5, PDEIVB
• CAS Number: 1653990-78-3
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 457-619 nmol/min·mg
• mol wt: ~78 kDa
• NCBI accession no.: NM_002600
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PDE4B(5142)

Description

General description

PDE4B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and has homology to the ′dunce′ gene of Drosophila. The ′dunce′ gene is one of several genes critical for normal learning and memory in the fly. Altered activity of PDE4B has been associated with schizophrenia and bipolar affective disorder. DISC1 interacts with the UCR2 domain of PDE4B and elevation of cellular cAMP leads to dissociation of PDE4B from DISC1 and in increase in PDE4B activity. PDE4B gene itself does not link to major depressive disorder (MDD) but elevated mRNA levels of PDE4B have been implicated in the pathophysiology of MDD.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable