SRP0124

CoREST human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

• Synonym(s): RCOR1, REST corepressor 1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥60% (SDS-PAGE)
• form: aqueous solution
• mol wt: 20 kDa
• packaging: pkg of 100 μg
• NCBI accession no.: NM_015156
• UniProt accession no.: Q9UKL0
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RCOR1(23186)

Description

General description

CoREST (also referred to as RCOR1 (REST corepressor 1)) mRNA is ubiquitously expressed in all tissues. It is a corepressor of the RE1-silencing transcription factor REST. In nonneuronal cells, the transcription factor binds to RE1 neuron-restrictive silencer elements and suppresses gene expression. CoREST is a component of the BRAF35 (BRCA2-associated factor 35)–histone deacetylase complex. The RCOR1 gene is mapped to human chromosome 14q32.
Human recombinant CoREST, GenBank Accession No. NM_015156), amino acids 305-end, with N-terminal His tag, MW = 20kDa, expressed in Escherichia coli expression system.

Application

Useful for the stimulation of LSD1 enzyme activity

Biochem/physiol Actions

CoREST associates with REST (RE1-silencing transcription factor), the histone deacetylases 1 and 2 (HDAC1/2), and LSD1 (lysine-specific histone demethylase 1A). This complex is involved in chromatin remodeling for changing gene expression. The LSD1/CoREST/REST compressor complex is also required for the expression of Herpes Simplex virus genes. LSD1 along with CoREST is responsible for the oxidative demethylation of the H3 histone (mono- and di-methylated Lysine4 residue). Deletions in the CoREST gene results in unfavorable clinical outcomes in DLBCL (diffuse large B-cell lymphoma) patients. It might also participate in the epigenetic control of blood cell development.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.