SAB4200344

Anti-SMYD3 antibody, Mouse monoclonal

clone SMYD3-2, purified from hybridoma cell culture

• Synonym(s): Anti-KMT3E, Anti-SET and MYND domain containing 3, Anti-ZMYND1, Anti-Zinc finger MYND domain-containing protein 1, Anti-Zinc finger protein, subfamily 3A (MYND domain containing), 1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SMYD3-2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~43 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 5-10 μg/mL using whole extracts of HeLa cells
• isotype: IgG1
• UniProt accession no.: Q9H7B4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SMYD3(64754) ; mouse ... Smyd3(69726) ; rat ... Smyd3(498295)

Description

General description

Monoclonal Anti-SMYD3 (mouse IgG1 isotype) is derived from the hybridoma SMYD3-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human SMYD3. SMYD3 belongs to the SMYD family of chromatin regulators. It is a histone methyltransferase, that plays a role in transcriptional regulation as a member of an RNA polymerase complex. SMYD3 contains a SET domain, which is critical for the methylation of histone H3 at Lys⁴ and a myeloid, Nervy, and DEAF-1 (MYND)-type zinc-finger domain, which is common to developmental proteins.

Immunogen

Fusion protein corresponding to a fragment of human SMYD3.

Application

Monoclonal Anti-SMYD3 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

SMYD3 is important in heart and skeletal muscle development. Overexpression of SMYD3 is observed in hepatocellular cancer, colorectal cancer and breast cancer. SMYD3 is essential for estrogen receptor-regulated gene transcription in the estrogen signaling pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable