SAB4200332

Anti-phospho-CD3ζ (pTyr¹¹¹) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CD247, Anti-CD3H, Anti-CD3Q, Anti-CD3Z, Anti-T cell receptor T3 zeta chain, Anti-T3Z, Anti-TCRK, Anti-Tcrz
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.; western blot: 1-2 μg/mL using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
• UniProt accession no.: P24161
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr111)
• Gene Information: mouse ... CD247(12503)

Description

General description

T-cell surface glycoprotein CD3 zeta chain (CD3ζ), also called CD247, is a component of T-cell antigen receptor (TCR) multimeric complex. CD3ζ comprises three immunoreceptor tyrosine-based activation motifs (ITAMs) with conserved Tyr-X-X-Leu/lle sequence, short ectodomain of nine amino acids and a long cytoplasmic tail region. CD247 gene is mapped to human chromosome 1q24.2 and is majorly expressed in the natural killer and T cells.

Specificity

Anti-phospho-CD3z(pTyr111) recognizes mouse phosphorylatedCD3z(pTyr111).

Immunogen

synthetic phosphopeptide corresponding to residues surrounding Tyr¹¹¹ of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by two amino acids in human.

Application

Anti-phospho-CD3ζ (pTyr¹¹¹) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

T-cell surface glycoprotein CD3 zeta chain (CD3ζ orCD3z) signal peptide in the N-terminal region aids in targeting protein translation to the endoplasmic reticulum. It is involved in the activation of the T cells and plays a key role in the recognition of antigenic peptide on antigen-presenting cells or tumor cells. CD3z chain plays a key role in regulating the TCR assembly, transport, and cell surface expression. CD3ζ reorganizes the actin-based cytoskeleton and regulates the morphology of immune cells. Downregulation of CD247also impacts the expression of the zeta chain Of T cell receptor associated protein kinase 70 (ZAP70) of the T-cell signal-transduction pathway. Lack of CD3ζ impairs retinal ganglion cell (RGC) motility and its presence is crucial for synaptic wiring in the retina. The absence of CD3ζ in T cells is postulated to be involved in the pathology of rheumatoid arthritis, systemic lupus erythematosus (SLE) and cancer. It is regarded as a potential therapeutic target for ovarian cancer treatment.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable