SAB4200145

Anti-AMSH-LP/STAMBPL1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-ALMalpha, Anti-AMSH-FP, Anti-AMSH-like protease, Anti-STAM binding protein-like 1, Anti-associated molecule with the SH3 domain of STAM (AMSH) like protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~50 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2-5 μg/mL using human HeLa cells; western blot: 1-2 μg/mL using whole extracts of human PMC2 cells
• UniProt accession no.: Q96FJ0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STAMBPL1(57559)

Description

General description

STAM binding protein like 1 (STAMBPL1) or AMSH-LP (AMSH-like protein), is a close homolog of AMSH (Associated Molecule with the SH3 domain of STAM). AMSH and AMSH-LP belong to the Joseph disease protease and JAB1/MPN/Mov34 metalloenzyme (JAMM) domain metalloprotease family of Zn²⁺-dependent deubiquitinating enzymes (DUBs). STAMBPL1 is mapped to human chromosome 10q23.31.

Specificity

Anti-AMSH-LP/STAMBPL1 recognizes human AMSH-LP/STAMBPL1.

Application

Anti-AMSH-LP/STAMBPL1 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

STAM binding protein like 1 (STAMBPL1), along with Associated Molecule with the SRC homology 3 domain (SH3) of STAM (AMSH proteins) mediate the deubiquitination of endosomal proteins and specifically cleave lysine-63-linked polyubiquitin chains. AMSH-LP, like AMSH, interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. However, AMSH-LP, unlike AMSH, fails to bind to the SH3 domain of STAM, suggesting that they are not functionally redundant. AMSH-LP positively regulates transforming growth factor (TGF-β) signaling through interaction with inhibitory I-SMADs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable