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SAB4200102

Sigma-Aldrich

Anti-AS160 (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL

Synonym(s):

Anti-Akt substrate of 160 kDa, Anti-TBC1D4 (TBC1 domain family, member 4)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~160 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells overexpressing human AS160

UniProt accession no.

O60343

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TBC1D4(9882)

General description

AS160 is a protein kinase B (Akt) substrate of 160 kDa, also known as TBC1D4. This gene is mapped to human chromosome 13q22.2 and encodes a Rab guanosine triphosphatase (GTPase)-activating protein (RabGAP). It has a number of insulin-regulatable protein kinase B (AKT) phosphomotifs. AS160 is expressed in many differentiated tissues such as adipocytes and skeletal muscles.

Application

Anti-AS160 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

AS160 (protein kinase B (Akt) substrate) is known to play an inhibitory role in the transport of glucose transporter 4 (GLUT4) vesicles. AS160 is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. Reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Insulin-induced effects on the subcellular localization of AKT1, AKT2 and AS160 in rat skeletal muscle
Zheng X and Cartee GD
Scientific Reports, 6(4), 39230-39230 (2016)
A method to identify serine kinase substrates Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain
Kane S, et al.
The Journal of biological chemistry, 277(25), 22115-22118 (2002)
Identification of a novel AS160 splice variant that regulates GLUT4 translocation and glucose-uptake in rat muscle cells
Baus D, et al.
Cellular Signalling, 20(12), 2237-2246 (2008)
Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities
Hatakeyama H, et al.
The Journal of Biological Chemistry, 294(4), 1161-1172 (2019)
Regulation of sarcolemmal glucose and fatty acid transporters in cardiac disease
Schwenk RW, et al.
Cardiovascular Research, 79(2), 249-258 (2008)
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