R3400

Monoclonal Anti-c-K-Ras antibody produced in mouse

clone 234-4.2, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD00239650
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 234-4.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 21 kDa
• species reactivity: mouse, human, rat
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10 μg/mL using normal skin tissue; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2κ
• UniProt accession no.: P01116
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... KRAS(3845) ; mouse ... Kras(16653) ; rat ... Kras(24525)

Description

General description

c-K-Ras or KRAS (kirsten rat sarcoma-2 virus oncogene) is a small GTP-binding protein. It is located on human chromosome 12p12.1. KRAS has two splice variants, such as KRASA and KRASB (KRAS proto-oncogenes). Expression of KRASA is restricted to specific tissues whereas KRASB is expressed everywhere .

Specificity

The antibody reacts with c-K-Ras and v-K-Ras p21. It crossreacts with cH-Ras and cN-Ras p21 on immunoblots.

Immunogen

recombinant p21 protein

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

KRAS (kirsten rat sarcoma-2 virus oncogene) mutation leads to poor prognosis of early rectal cancer.

Physical form

Solution in 0.05 M sodium phosphate buffer, pH7.5, containing 0.2% gelatin and < 0.1% sodium azide

Preparation Note

Purified using protein A or G

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves