P6101
Anti-Platelet-Derived Growth Factor antibody produced in goat
IgG fraction of antiserum, lyophilized powder
Synonym(s):
Anti-PDGF
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About This Item
biological source
goat
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: suitable
neutralization: suitable
western blot: suitable
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PDGFA(5154) , PDGFB(5155)
General description
Platelet-derived growth factor (PDGF) is a serum growth factor purified from human platelets. The members of PDGF are homodimers of A-, B-, C- and –D polypeptide chains and an AB heterodimer. PDGF is a mitogen and stimulates the proliferation of cells of connective tissue and glial cells, survival, differentiation, chemotaxis and wound healing. The cellular effects of PDGF isoforms are mediated by binding to α- and β-receptors which leads to activation of downstream signaling via PI3K, STAT and Ras/MAPK. Overexpression of PDGF isoforms has been observed in various malignancies including gliomas, osetosarcoma, chronic myelomonocytic leukemia and non-small-cell lung cancers
Anti-Human Platelet-Derived Growth Factor (PDGF) specifically recognizes natural human PDGF. It will also neutralize the activity of recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. It shows cross reactivity with recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB.
Anti-Human Platelet-Derived Growth Factor (PDGF) specifically recognizes natural human PDGF. It will also neutralize the activity of recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB. It shows cross reactivity with recombinant human PDGF-AB, recombinant human PDGF-AA, recombinant human PDGF-BB, and natural porcine PDGF-BB.
Specificity
The antibody will neutralize the biological activity of natural human PDGF, recombinant human PDGF-AB, PDGF-AA and PDGF-BB and natural porcine PDGF-BB.
Immunogen
natural human PDGF.
Application
Anti-Human Platelet-Derived Growth Factor (PDGF) antibody may be used for ELISA at a working concentration of 0.25 μg/ml. The antibody has been used for ELISA at a dilution of 1:500 using human serum.. For immunoblotting, a concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions and has been used to neutralize PDGF of primary Human dermal fibroblasts (HDFs) and human platelet-enriched plasma.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) with 5% trehalose.
Preparation Note
Purified using protein G.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Fabio Celotti et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 14(2), 195-202 (2006-04-25)
Platelet-enriched plasma (PRP) is used in therapy as a source of growth factors in bone fracture and wound healing; however, few data exist on its role in the different aspects of the healing process. The effect of PRP and of
PDGF and PDGF receptors in glioma
Nazarenko I et al
Upsala Journal of Medical Sciences, 112, 99-112 (2012)
Robyn R Mercer et al.
Experimental cell research, 310(2), 270-281 (2005-09-13)
Breast cancer frequently metastasizes to bone, resulting in osteolytic lesions. These lesions, formed by activated osteoclasts, cause pain, an increased susceptibility to fractures, and hypercalcemia. It has been shown that breast cancer cells communicate with osteoblasts and subsequently stimulate osteoclast
David J Margolis et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(10), 1822-1829 (2009-07-30)
Venous leg ulcers are a prevalent nonhealing wound of the lower extremity. Although topically applied growth factors successfully improve wound repair in animal studies, similar studies on humans with venous leg ulcers have not been successful. This study was designed
Nanna Lindberg et al.
Upsala journal of medical sciences, 117(2), 92-98 (2012-03-02)
Platelet-derived growth factor B (PDGF-B) is a growth factor promoting and regulating cell migration, proliferation, and differentiation, involved in both developmental processes and in maintaining tissue homeostasis under strict regulation. What are the implications of prolonged or uncontrolled growth factor
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