MAK210

Human Calpain 1 Inhibitor Screening Kit

Sufficient for 100 Fluorometric tests

• UNSPSC Code: 12352200
• NACRES: NA.25

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• relevant disease(s): cardiovascular diseases; cancer
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CAPN1(823)

Description

General description

Mammalian calpains are calcium-activated, neutral, non-lysosomal, intracellular proteases that may be ubiquitous, stomach-specific, or muscle-specific. Calpain 1 is a ubiquitous protease that is involved in calcium homeostasis and the regulation of apoptosis. In cardiomyocytes, activated calpain 1 induces apoptosis by BID cleavage and release of cytochrome c from the mitochondria.

The Human Calpain 1 Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high-throughput screening of Calpain 1 inhibitors. Calpain 1 activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate (Ac-LLY-AFC) to yield AFC, a fluorescent product (λ_ex = 400/λ_em = 505 nm), proportional to the enzymatic activity present.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the screening of inhibitors of human Calpain 1.

Principle

The Human Calpain 1 Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high-throughput screening of Calpain 1 inhibitors. Calpain 1 activity is measured by cleaving a synthetic 7-amino-4-trifluoromethylcoumarin (AFC)-based substrate (Ac-LLY-AFC) to yield AFC, a fluorescent product (λ_ex = 400/λ_em = 505 nm), proportional to the enzymatic activity present.

Kit Components Only

• Assay Buffer
• Substrate
• Calpain 1 Enzyme
• Inhibitor Control

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup