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MAK057

Sigma-Aldrich

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Citrate Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Citrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.

Application

Citrate Assay kit has been used to determine the concentration of citrate in samples.

Suitability

Suitable for the measurement of citrate in a variety of samples including tissue and cells

Principle

Citrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 μM-10 mM) of citrate in a variety of samples.

related product

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Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

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