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L2158

Sigma-Aldrich

L-Leucine p-nitroanilide hydrochloride

chromogenic, ≥98% (HPLC), powder

Synonym(s):

H-Leu-pNA

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About This Item

Empirical Formula (Hill Notation):
C12H17N3O3 · HCl
CAS Number:
16010-98-3
Molecular Weight:
287.74
MDL number:
MFCD00058248
UNSPSC Code:
12352204
PubChem Substance ID:
24896304
NACRES:
NA.28

Product Name

L-Leucine p-nitroanilide hydrochloride, ≥98% (HPLC)

Quality Level

300

Assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL

storage temp.

2-8°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C12H17N3O3.ClH/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18;/h3-6,8,11H,7,13H2,1-2H3,(H,14,16);1H/t11-;/m0./s1

InChI key

PFOYXOWPGRWPLS-MERQFXBCSA-N

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Application

L-Leucine p-nitroanilide hydrochloride has been used as a substrate to measure the activity of leucine aminopeptidase (LAP):
  • for spectrophotometric assays
  • in the intestinal samples, such as duodenum, jejunum, and ileum
  • in the hemolymph of Manila clam

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCF7922
SLBZ4137
SLBR4028V
SLBK2430V
SLBL4023V

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Susan E Ford et al.
Fish & shellfish immunology, 23(2), 280-291 (2006-12-19)
Components of the haemolymph are understood to constitute the internal defense system of bivalve mollusks and their levels are often considered to be indicators of "health"; however, relatively little proof exists of the role that these elements play in the
J Williams et al.
British poultry science, 49(3), 329-339 (2008-06-24)
1. The objective of this experiment was to study two feeding methods, which could potentially act on the gut microflora, the structure and/or the function of the digestive tract and thereby improve the performance of broilers. 2. Four dietary treatments

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