I5279
IgG2, Lambda from human myeloma plasma
purified immunoglobulin, >95% (SDS-PAGE)
Synonym(s):
Human IgG2-λ
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About This Item
Recommended Products
biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
Assay
>95% (SDS-PAGE)
shipped in
dry ice
storage temp.
−20°C
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General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Human myeloma IgG2, λ is purified from human plasma by fractionation, ion-exchange and affinity chromatography procedures.
Human myeloma IgG2, λ is purified from human plasma by fractionation, ion-exchange and affinity chromatography procedures.
Application
The purified IgG2, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG2, λ was used to check the efficiency of hydrophobic interaction membrane chromatography (HIMC).
Physical form
Solution in 0.02 M Tris buffered saline, pH 8.0.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Lu Wang et al.
Analytical chemistry, 82(1), 452-455 (2009-12-17)
Human immunoglobulin G (IgG) consists of four subclasses, each having specific biological functions and physical properties. Fractionation of these subclasses is challenging, both at analytical and preparative scales. This paper examines the feasibility of separating the major IgG subclasses, i.e.
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG
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