CMC0008

STEADY Chemically Competent Cells

for plasmid production using unstable DNA

• UNSPSC Code: 12352200

Properties

• biological source: Escherichia coli
• grade: for molecular biology
• form: buffered aqueous solution
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Features and Benefits

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

Components

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning