TGP 54

94022430

• UNSPSC Code: 41106514

Properties

• biological source: mouse pancreas
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Somatostatin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse pancreatic islet cell tumour

Cell Line Description

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

Application

Study of exocrine pancreas carcinomas, hormone secretion studies.

Culture Medium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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