F10-89-4
89062101, Reactivity: Anti-CD45 determinant 1, human
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About This Item
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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS1/1
Reactivity
Anti-CD45 determinant 1, human
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS1/1 x Mouse BALB/c spleen
Cell Line Description
The antibody recognises the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognised by F10-89-4 is a glycoprotein with a molecular weight of 190kDa to 215kDa.
Immunogen
Human T lymphocytes
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation
Other Notes
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